MilleGen proposes a gene synthesis service to accelerate your research.
100% sequence-verification utilizing rigorous quality standards and state-of-the art sequencing technology
Optional codon optimization for gene expression
Subcloning in pUC19 vector included; delivered as plasmid DNA
Data package including optimized sequence and sequence data with raw trace files
Standard Constructed Clones up to 10 kb Simply provide with a DNA sequence and we will deliver a sequence-verified synthetic gene clone.
Complex Constructed Clones Certain gene sequences may require more complex and sophisticated manufacturing procedures. Characteristics of complex gene sequences may include the following:
- High or low GC content - High AT content - Highly repetitive regions - Homopolymers
A large vector family allows to propose the best solution to clone your gene :
Plasmidic vectors
Multicistronic vectors with viral or eucaryotic IRES
According to your needs, we will help you to determine the adapted vector : with our without reporter gene, eukaryotic resistance gene, coexpression of several genes, promoter cloning downstream of a reporter gene for the study of this promoter, cloning one or several gene of interest upstream of a strong promoter, cloning genes of interest in fusion with a tag in an expression vector...
MilleGen cloning and vector department offers to engineer a simple and rapid cloning strategy for the achievement of the required construction.
Any mutation, including single or multiple site deletions, insertions, and substitutions can be introduced by site-directed mutagenesis into a customer’s gene of interest.
