MilleGen has acquired an extensive experience on large sequencing projects (>96 sequences to whole genome projects):
EST and SAGE library
Plasmids
PCR products
BAC / PAC and Cosmid end
Our capacities guarantee ultra fast and fluent sample processing of even the largest number of samples simultaneously. Samples can be supplied as either purified DNA or bacterial cultures in 96 or 384 well formats. The all sequencing process is realised on a complete robotic platform.
DNA from bacterial cultures or PCR products are purified in 96 well microplates using a robotic biosystem (Tecan Genesis RSP200 and MWG Robotseq 2500). The fluorescent labelled sequencing products generated on a MWG Robotseq 2500 are separated by electrophoresis on 96 capillary sequencers (ABI 3730XL).
The HTS platform could achieve an average read up to to 1000 bp (depending on the type of organism, the cloning strategy or the sequence direction). We offer highest capacities and shortest turn around times.
Plasmid extraction and sequencing of 10,000 clones is done easily within 10 working days.
An analysis for each read using the Phred/Phrap/Consed software package allows determining the quality of the DNA sequencing runs. This bioinformatic analysis has been automated and a report is placed in your download directory.
A classic report contains the "Score " representing the number of high quality base calls (>=99%) for each read. You could also order a complete report with a more detailed statistical analysis.
All sequences data and chromatograms are available to researchers through our secure web interface.
