Whether you require plasmid DNA for in vitro, in vivo or pre-clinical studies, MilleGen will customise his service for you according to the production scale and the purification grade.
Five selections of the production scale
Plasmid DNA preparation
Expected yield*
Material
References
Miniprep
5-10 µg
Glycerol stock or fresh culture on LB agar
SADN 0001
Midiprep
50-100 µg
Glycerol stock or fresh culture on LB agar
SMID 0001
Maxiprep
250-500 µg
Glycerol stock or fresh culture on LB agar
SMAX 0001
Megaprep
1-2.5 mg
Glycerol stock or fresh culture on LB agar
SMEG 0001
Gigaprep
5-10 mg
Glycerol stock or fresh culture on LB agar
SGIG 0001
*Actual yields depend on plasmid copy number, size of insert and host strain.
Three purification grades of plasmid DNA in order to supply the most suitable and ready-to-use products
Molecular biology research grade The plasmid DNA purity provides excellent results in most molecular biology applications: cloning, sequencing, in vitro transcription, restriction analysis, and transfection
Endotoxin-free grade
Endotoxin yield, less than 0.1 EU/mg plasmid DNA (FDA specifications) is well suited for highly reproducible and efficient transfection. Endotoxins (LPS) are released during lysis step of plasmid purification. They significantly reduce transfection efficiencies and induce non-specific activation of immune responses in immune cells, which can lead to misinterpretation of transfection result.
Pre-clinical grade
Protein level is less than 0.1 EU/mg and the purification is guaranteed with an absence of microorganisms (Bioburden assay verification).
MilleGen offers a stringent quality control from start to finish of plasmid DNA production procedure. Each batch of plasmid DNA is delivered with a certificate of analysis.
