Publications    

Combinatorial engineering to enhance thermostability of amylosucrase.
 
Protein Sci. 2008, Apr 25. [Epub ahead of print]. PMID:18441231
 
Emond S^1,2, André I¹, Jaziri K¹, Potocki-Veronese G¹, Mondon P², Bouayadi K², Kharrat H², Monsan P¹ , Remaud.Simeon M¹.
 
¹ Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP),  INSA, 135 avenue de Rangueil, 31077 Toulouse cedex 4, France.

² MilleGen SA, Immeuble Biostep-Bat A, rue Pierre et Marie Curie, BP 38183, 31681 Labège cedex, France

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A novel random mutagenesis approach using human mutagenic DNA polymerases to generate enzyme variant libraries.
 
Protein Eng. Des. Sel. 2008, 21:267-274. PMID:18287177
 
Emond S^1,2, Mondon P², Pizzut-Serrin S¹ , Douchy L², Crozet F², Bouayadi K², Kharrat H², Potocki-Veronese G¹ , Monsan P¹ , Remaud.Simeon M¹.
 
¹ Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP),  INSA, 135 avenue de Rangueil, 31077 Toulouse cedex 4, France.

² MilleGen SA, Immeuble Biostep-Bat A, rue Pierre et Marie Curie, BP 38183, 31681 Labège cedex, France

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Human antibody libraries: A race to engineer and explore a larger diversity.
 
Front. Biosci. 2008, 13:1117-1129. PMID: 17981617 
 
Mondon P, Dubreuil O, Bouayadi K and Kharrat H.
 
MilleGen SA, Immeuble Biostep-Bat A, rue Pierre et Marie Curie, BP 38183, 31681 Labège cedex, France.

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Optimized and automated protocols for high throughput screening of amylosucrase libraries.   J. Biomol. Screen. 2007, 12:715-723. PMID: 17517906   Stéphane Emond1,2, Gabrielle Potocki-Véronèse1, Philippe Mondon2, Khalil Bouayadi2, Hakim Kharrat2, Pierre Monsan1 and Magali Remaud-Simeon1.   1 Laboratoire Biotechnologie-Bioprocédés, UMR CNRS 5504, UMR INRA 792, INSA, 135 avenue de Rangueil, 31077 Toulouse cedex 4, France. 2 MilleGen SA, Immeuble Biostep-Bat A, rue Pierre et Marie Curie, BP 38183, 31681 Labège cedex, France

Method for generation of human hyperdiversified antibody fragment library.   Biotechnol. J. 2007, 2:76-82. PMID: 17225253   Mondon P, Souyris N, Douchy L, Crozet F, Bouayadi K and Kharrat H.   MilleGen SA, Immeuble Biostep-Bat A, rue Pierre et Marie Curie, BP 38183, 31681 Labège cedex, France

Translational induction of VEGF Internal ribosome entry site elements during the early response to ischemic stress. Circ Res. 2007 Feb 16;100(3):305-8. PMID: 17255526 Bornes S*, Prado-Lourenco L*,•, Bastide A*, Zanibellato C*, Iacovoni JS*, Lacazette E*,  Prats AC*, Touriol C*, Prats H* *INSERM P U858, Université Paul Sabatier, Toulouse, France • MilleGen SA, Labège, France

Syntheses of functionalized biotin N-1' derivatives: New tools for the control of gene expression with small molecules.   Bioconjugate Chem. 2006, 17 :1030 -1035. PMID: 16848412   Inard C*, Fourcade E, Baron R, Tovar D, Laurent Chaisemartin L, Blonski C, and  Faye JC.   INSERM U563, C. P. T. P., Département Innovation Thérapeutique et Oncologie Moléculaire, Institut Claudius Regaud, Toulouse France Laboratoire de Synthèse et Physicochimie de Molécules d'Intérêt Biologique (LSPCMIB), ESA 5068, Université Paul Sabatier, Toulouse France *MilleGen SA, Immeuble Biostep-Bat A, rue Pierre et Marie Curie, BP 38183, 31681 Labège cedex, France

Testosterone regulates FGF-2 expression during testis maturation by an IRES-dependent translational mechanism.   FASEB J. 2006, 20:476-8. PMID: 16423876 Gonzalez-Herrera Irma G.*,†,1 Leonel Prado-Lourenco,*,†, ‡,1 Frédéric Pileur,*,† Caroline Conte,*,† Aurélie Morin,• Florence Cabon,‡ Hervé Prats,*,† Stephan Vagner,*,†Francis Bayard,*,†,║ Sylvie Audigier,*,† and Anne-Catherine Prats*,† *INSERM U589, Toulouse, France †UniversitéPaul Sabatier, Toulouse France •Laboratoire Oncogenèse, Différenciation et Transduction du Signal”, Villejuif, France ║Centre Hospitalier Universitaire de Rangueil, Toulouse, France ‡MilleGen SA, Labège, France

IRES-dependent regulation of FGF-2 mRNA translation in pathophysiological conditions in the mouse.   Biochem Soc Trans. 2006 34:17-21. PMID: 16246170   Gonzalez-Herrera IG, Prado-Lourenco L*, Teshima-Kondo S, Kondo K, Cabon F, Arnal JF, Bayard F and Prats AC.   INSERM U589, "Hormones, facteurs de croissance et physiopathologie vasculaire", Institut Louis Bugnard, IFR31, CHU Rangueil, Toulouse,France *MilleGen SA, Immeuble Biostep-Bat A, rue Pierre et Marie Curie, BP 38183, 31681 Labège cedex, France

Chemical-based translational induction of luciferase expression: an efficient tool for in vivo screening of protein farnesylation inhibitors.   Mol Pharmacol. 2005, 67:1829-33. PMID: 15784845   Boijoux O 1, Boutonnet C 3, 4, Giamarchi C 2, Favre G 2, Vagner S 3 and Faye JC 1. 1 INSERM U563, Centre de Physiopathologie Toulouse Purpan, Toulouse, France 2 Institut Claudius Regaud Toulouse, Université Paul Sabatier, Toulouse, France 3 INSERM U 589, Centre Hospitalier Universitaire Rangueil, Toulouse, France 4 MilleGen SA, Labège, France

Technologies of human recombinant antibodies.   Biofutur 2005, 258:34-40.   Mondon P, Dubreuil O, Bouayadi K and Kharrat H.   MilleGen SA, Immeuble Biostep-Bat A, rue Pierre et Marie Curie, BP 38183, 31681 Labège cedex, France

Pharmacological-based translational induction of transgene expression in mammalian cells.   EMBO Rep. 2004, 5:721-7. PMID: 15192697   Boutonnet C 1,2, Boijoux O 3, Bernat S 2, Kharrat A 2, Favre G 3, Faye JC 3, Vagner S 1. 1INSERM U589, Institut Louis Bugnard, CHU Rangueil Toulouse, France 2MILLEGEN SA, Labège, France 3INSERM U563, Centre Claudius Régaud, Toulouse, France

Hyperglycemia upregulates translation of the fibroblast growth factor 2 mRNA in mouse aorta via internal ribosome entry site.   FASEB J. 2004, 18:1583-5. PMID: 15289445   Teshima-Kondo S, † Kondo K, † Prado-Lourenco L, *,‡ Gonzalez-Herrera IG, * Rokutan K, † Bayard F, Arnal JF * and Prats AC *.   *INSERM U589, Institut Louis Bugnard, IFR31, CHU Rangueil, Toulouse, France †Department of Stress Science, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima 770-8503, Japan ‡ MILLEGEN SA, Labège, France