Monoclonal antibody cloning from hybridoma (mice, rat)    


The development of genetic engineering techniques has enabled the cloning
and sequencing of variable genes coding for the heavy (VH) and light (VL)
chains of the monoclonal antibody (mAb). The sequencing of the VH and VL
retains and immortalizes the mAb which can be crucial for the rescue of
unstable hybridoma cell lines. Furthermore, an antibody can be identified by the DNA sequence thus strengthening its patent rights.


MilleGen has developed a very efficient cloning technique of all the variable genes of the heavy (VH) and light (VL) chains from a hybridoma cell line. The antigen-binding ability of an antibody is usually conserved when only the VH and VL domains are used. The isolated VH and VL will be engineered to produce the Fab. Testing the binding properties of the recombinant antibody can be used as a tool to validate the VH and VL sequences coding for the monoclonal antibody. The antibody fragment can then be used to optimize the immune properties (see genetic engineering of antibody).